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wnt3a growth factor  (PeproTech)


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    Structured Review

    PeproTech wnt3a growth factor
    Wnt3a Growth Factor, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3a+growth+factor/wnt3a+protein/pmc10091881__thnov13p1949s1-55-40-41
    Average 90 stars, based on 1 article reviews
    wnt3a growth factor - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
    Article Snippet: Briefly, frozen enteroids were thawed, washed, fragmented and suspended in ice-cold Matrigel (CorningTM) supplemented with the recombinant human growth factors Noggin (100 ng/mL; Peprotech), R-spondin (500 ng/mL; Peprotech), EGF (50 ng/mL; CorningTM), Wnt3a (100 ng/mL; Peprotech), Y-27632 (10 μM; Peprotech), SB202190 (10 μM; Tocris BioscienceTM), LY2157299 (500 nM; A ChemBlock) and CHIR99021 (2.5 μM; Tocris BioscienceTM).

    Article Title: TGFβ2-Driven Ferritin Degradation and Subsequent Ferroptosis Underlie Salivary Gland Dysfunction in Postmenopausal Conditions.
    Article Snippet: Wnt3a (100 ng mL−1; Peprotech) was added to growth media at early passages (P0–P2) to enhance stability, while SB was omitted for TGFβ2 treatment experiments.

    Article Title: Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration
    Article Snippet: The culture medium was changed every 3 days using EEO medium composed of DMEM/F12 (Gibco), penicillin/streptomycin (100 U/L; Gibco), HEPES (10 mM; Gibco), GlutaMAX (1×; Gibco) , B27 supplement (1×; Gibco), N2 supplement (1×; Gibco), ITS Liquid Media Supplement (1×; Sigma‒Aldrich), Nicotinamide (1 mM; Sigma‒Aldrich), N-acetylcysteine-1 (1.25 mM; Sigma‒Aldrich), EGF (50 ng/mL; Peprotech), FGF-10 (100 ng/mL; Peprotech), Wnt3a (200 ng/mL; Peprotech), Noggin (100 ng/mL; Peprotech), Rspondin-1 (200 ng/mL; Peprotech), Y-27632 (9 μM; AbMole BioScience), A83-01 (500 nM; AbMole BioScience), SB202190 (10 μM; AbMole BioScience).

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses
    Article Snippet: EBs were treated with 5 ng ml −1 BMP4 for 1 d and then 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 6 ng ml −1 Activin A (PeproTech), 6 ng ml −1 WNT3A (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 25 ng ml −1 follistatin (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Article Title: NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.
    Article Snippet: This medium was enriched with various growth factors and supplements: 50 ng/mL epidermal growth factor (Sigma-Aldrich), 100 ng/mL FGF10 (Peprotech), 100 ng/mL Wnt3a (Peprotech, Rocky Hill, NJ), 100 ng/mL Noggin (R&D Systems), 1 mg/mL R-spondin 1 (Antibody and Protein Resource at Vanderbilt, Palo Alto, CA), 13 B27 supplement (Gibco Media), 10 nmol/L gastrin (Sigma-Aldrich), 1 mmol/L N-acetylcysteine (Sigma-Aldrich), 13HEPES buffer (Gibco Media), and 13N2 supplement (GibcoMedia).

    Article Title:
    Article Snippet: Related to Figure 1C, D. Experiment number (stage 1) 1 2 3 4 5 St ag e 1 a BE1 + 0.1% BSA 1 day 100 ng/mL Activin A (Peprotech) 2 μM CHIR99021 (Axon MedChem) 3 μM CHIR 20 ng/μL Wnt3A (PeproTech) St ag e 1 b BE1 + 0.1% BSA 2 days 3 days 100 ng/mL Activin A 5 ng/mL FGF2 (Novoprotein) Supplemental Table S2.

    Article Title: BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways
    Article Snippet: After Matrigel polymerization for 10 min at 37 °C, taste bud organoid culture medium based on DMEM/F12 medium (Gibco) supplemented with Wnt3a (100 ng/mL, PeproTech, Cranbury, NJ, USA), R-spondin-1 (200 ng/mL, R&D systems, Minneapolis, MN, USA), Noggin (100 ng/mL, PeproTech), Y27632 (10 μM), N-acetylcysteine (1 mM, Sigma), epidermal growth factor (50 ng/mL, PeproTech), N2 (1%, Gibco), and B27 (2%, Gibco) was added to the plate and replaced every 3 days.

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses.
    Article Snippet: EBs were treated with 5 ng ml−1 BMP4 for 1 d and then 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 6 ng ml−1 Activin A (PeproTech), 6 ng ml−1 WNT3A (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 25 ng ml−1 follistatin (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Cell Culture:

    Article Title: Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
    Article Snippet: Briefly, frozen enteroids were thawed, washed, fragmented and suspended in ice-cold Matrigel (CorningTM) supplemented with the recombinant human growth factors Noggin (100 ng/mL; Peprotech), R-spondin (500 ng/mL; Peprotech), EGF (50 ng/mL; CorningTM), Wnt3a (100 ng/mL; Peprotech), Y-27632 (10 μM; Peprotech), SB202190 (10 μM; Tocris BioscienceTM), LY2157299 (500 nM; A ChemBlock) and CHIR99021 (2.5 μM; Tocris BioscienceTM).

    Article Title: TGFβ2-Driven Ferritin Degradation and Subsequent Ferroptosis Underlie Salivary Gland Dysfunction in Postmenopausal Conditions.
    Article Snippet: Wnt3a (100 ng mL−1; Peprotech) was added to growth media at early passages (P0–P2) to enhance stability, while SB was omitted for TGFβ2 treatment experiments.

    Article Title: Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration
    Article Snippet: The culture medium was changed every 3 days using EEO medium composed of DMEM/F12 (Gibco), penicillin/streptomycin (100 U/L; Gibco), HEPES (10 mM; Gibco), GlutaMAX (1×; Gibco) , B27 supplement (1×; Gibco), N2 supplement (1×; Gibco), ITS Liquid Media Supplement (1×; Sigma‒Aldrich), Nicotinamide (1 mM; Sigma‒Aldrich), N-acetylcysteine-1 (1.25 mM; Sigma‒Aldrich), EGF (50 ng/mL; Peprotech), FGF-10 (100 ng/mL; Peprotech), Wnt3a (200 ng/mL; Peprotech), Noggin (100 ng/mL; Peprotech), Rspondin-1 (200 ng/mL; Peprotech), Y-27632 (9 μM; AbMole BioScience), A83-01 (500 nM; AbMole BioScience), SB202190 (10 μM; AbMole BioScience).

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses
    Article Snippet: EBs were treated with 5 ng ml −1 BMP4 for 1 d and then 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 6 ng ml −1 Activin A (PeproTech), 6 ng ml −1 WNT3A (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 25 ng ml −1 follistatin (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Article Title: NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.
    Article Snippet: This medium was enriched with various growth factors and supplements: 50 ng/mL epidermal growth factor (Sigma-Aldrich), 100 ng/mL FGF10 (Peprotech), 100 ng/mL Wnt3a (Peprotech, Rocky Hill, NJ), 100 ng/mL Noggin (R&D Systems), 1 mg/mL R-spondin 1 (Antibody and Protein Resource at Vanderbilt, Palo Alto, CA), 13 B27 supplement (Gibco Media), 10 nmol/L gastrin (Sigma-Aldrich), 1 mmol/L N-acetylcysteine (Sigma-Aldrich), 13HEPES buffer (Gibco Media), and 13N2 supplement (GibcoMedia).

    Article Title:
    Article Snippet: Related to Figure 1C, D. Experiment number (stage 1) 1 2 3 4 5 St ag e 1 a BE1 + 0.1% BSA 1 day 100 ng/mL Activin A (Peprotech) 2 μM CHIR99021 (Axon MedChem) 3 μM CHIR 20 ng/μL Wnt3A (PeproTech) St ag e 1 b BE1 + 0.1% BSA 2 days 3 days 100 ng/mL Activin A 5 ng/mL FGF2 (Novoprotein) Supplemental Table S2.

    Article Title: BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways
    Article Snippet: After Matrigel polymerization for 10 min at 37 °C, taste bud organoid culture medium based on DMEM/F12 medium (Gibco) supplemented with Wnt3a (100 ng/mL, PeproTech, Cranbury, NJ, USA), R-spondin-1 (200 ng/mL, R&D systems, Minneapolis, MN, USA), Noggin (100 ng/mL, PeproTech), Y27632 (10 μM), N-acetylcysteine (1 mM, Sigma), epidermal growth factor (50 ng/mL, PeproTech), N2 (1%, Gibco), and B27 (2%, Gibco) was added to the plate and replaced every 3 days.

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses.
    Article Snippet: EBs were treated with 5 ng ml−1 BMP4 for 1 d and then 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 6 ng ml−1 Activin A (PeproTech), 6 ng ml−1 WNT3A (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 25 ng ml−1 follistatin (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
    Article Snippet: Briefly, frozen enteroids were thawed, washed, fragmented and suspended in ice-cold Matrigel (CorningTM) supplemented with the recombinant human growth factors Noggin (100 ng/mL; Peprotech), R-spondin (500 ng/mL; Peprotech), EGF (50 ng/mL; CorningTM), Wnt3a (100 ng/mL; Peprotech), Y-27632 (10 μM; Peprotech), SB202190 (10 μM; Tocris BioscienceTM), LY2157299 (500 nM; A ChemBlock) and CHIR99021 (2.5 μM; Tocris BioscienceTM).

    Article Title: TGFβ2-Driven Ferritin Degradation and Subsequent Ferroptosis Underlie Salivary Gland Dysfunction in Postmenopausal Conditions.
    Article Snippet: Wnt3a (100 ng mL−1; Peprotech) was added to growth media at early passages (P0–P2) to enhance stability, while SB was omitted for TGFβ2 treatment experiments.

    Article Title: Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration
    Article Snippet: The culture medium was changed every 3 days using EEO medium composed of DMEM/F12 (Gibco), penicillin/streptomycin (100 U/L; Gibco), HEPES (10 mM; Gibco), GlutaMAX (1×; Gibco) , B27 supplement (1×; Gibco), N2 supplement (1×; Gibco), ITS Liquid Media Supplement (1×; Sigma‒Aldrich), Nicotinamide (1 mM; Sigma‒Aldrich), N-acetylcysteine-1 (1.25 mM; Sigma‒Aldrich), EGF (50 ng/mL; Peprotech), FGF-10 (100 ng/mL; Peprotech), Wnt3a (200 ng/mL; Peprotech), Noggin (100 ng/mL; Peprotech), Rspondin-1 (200 ng/mL; Peprotech), Y-27632 (9 μM; AbMole BioScience), A83-01 (500 nM; AbMole BioScience), SB202190 (10 μM; AbMole BioScience).

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses
    Article Snippet: EBs were treated with 5 ng ml −1 BMP4 for 1 d and then 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 6 ng ml −1 Activin A (PeproTech), 6 ng ml −1 WNT3A (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 25 ng ml −1 follistatin (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Article Title: NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.
    Article Snippet: This medium was enriched with various growth factors and supplements: 50 ng/mL epidermal growth factor (Sigma-Aldrich), 100 ng/mL FGF10 (Peprotech), 100 ng/mL Wnt3a (Peprotech, Rocky Hill, NJ), 100 ng/mL Noggin (R&D Systems), 1 mg/mL R-spondin 1 (Antibody and Protein Resource at Vanderbilt, Palo Alto, CA), 13 B27 supplement (Gibco Media), 10 nmol/L gastrin (Sigma-Aldrich), 1 mmol/L N-acetylcysteine (Sigma-Aldrich), 13HEPES buffer (Gibco Media), and 13N2 supplement (GibcoMedia).

    Article Title:
    Article Snippet: Related to Figure 1C, D. Experiment number (stage 1) 1 2 3 4 5 St ag e 1 a BE1 + 0.1% BSA 1 day 100 ng/mL Activin A (Peprotech) 2 μM CHIR99021 (Axon MedChem) 3 μM CHIR 20 ng/μL Wnt3A (PeproTech) St ag e 1 b BE1 + 0.1% BSA 2 days 3 days 100 ng/mL Activin A 5 ng/mL FGF2 (Novoprotein) Supplemental Table S2.

    Article Title: BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways
    Article Snippet: After Matrigel polymerization for 10 min at 37 °C, taste bud organoid culture medium based on DMEM/F12 medium (Gibco) supplemented with Wnt3a (100 ng/mL, PeproTech, Cranbury, NJ, USA), R-spondin-1 (200 ng/mL, R&D systems, Minneapolis, MN, USA), Noggin (100 ng/mL, PeproTech), Y27632 (10 μM), N-acetylcysteine (1 mM, Sigma), epidermal growth factor (50 ng/mL, PeproTech), N2 (1%, Gibco), and B27 (2%, Gibco) was added to the plate and replaced every 3 days.

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses.
    Article Snippet: EBs were treated with 5 ng ml−1 BMP4 for 1 d and then 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 6 ng ml−1 Activin A (PeproTech), 6 ng ml−1 WNT3A (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 25 ng ml−1 follistatin (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Lysis:

    Article Title: Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
    Article Snippet: Briefly, frozen enteroids were thawed, washed, fragmented and suspended in ice-cold Matrigel (CorningTM) supplemented with the recombinant human growth factors Noggin (100 ng/mL; Peprotech), R-spondin (500 ng/mL; Peprotech), EGF (50 ng/mL; CorningTM), Wnt3a (100 ng/mL; Peprotech), Y-27632 (10 μM; Peprotech), SB202190 (10 μM; Tocris BioscienceTM), LY2157299 (500 nM; A ChemBlock) and CHIR99021 (2.5 μM; Tocris BioscienceTM).

    Article Title: TGFβ2-Driven Ferritin Degradation and Subsequent Ferroptosis Underlie Salivary Gland Dysfunction in Postmenopausal Conditions.
    Article Snippet: Wnt3a (100 ng mL−1; Peprotech) was added to growth media at early passages (P0–P2) to enhance stability, while SB was omitted for TGFβ2 treatment experiments.

    Article Title: Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration
    Article Snippet: The culture medium was changed every 3 days using EEO medium composed of DMEM/F12 (Gibco), penicillin/streptomycin (100 U/L; Gibco), HEPES (10 mM; Gibco), GlutaMAX (1×; Gibco) , B27 supplement (1×; Gibco), N2 supplement (1×; Gibco), ITS Liquid Media Supplement (1×; Sigma‒Aldrich), Nicotinamide (1 mM; Sigma‒Aldrich), N-acetylcysteine-1 (1.25 mM; Sigma‒Aldrich), EGF (50 ng/mL; Peprotech), FGF-10 (100 ng/mL; Peprotech), Wnt3a (200 ng/mL; Peprotech), Noggin (100 ng/mL; Peprotech), Rspondin-1 (200 ng/mL; Peprotech), Y-27632 (9 μM; AbMole BioScience), A83-01 (500 nM; AbMole BioScience), SB202190 (10 μM; AbMole BioScience).

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses
    Article Snippet: EBs were treated with 5 ng ml −1 BMP4 for 1 d and then 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 6 ng ml −1 Activin A (PeproTech), 6 ng ml −1 WNT3A (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 25 ng ml −1 follistatin (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Article Title: NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.
    Article Snippet: This medium was enriched with various growth factors and supplements: 50 ng/mL epidermal growth factor (Sigma-Aldrich), 100 ng/mL FGF10 (Peprotech), 100 ng/mL Wnt3a (Peprotech, Rocky Hill, NJ), 100 ng/mL Noggin (R&D Systems), 1 mg/mL R-spondin 1 (Antibody and Protein Resource at Vanderbilt, Palo Alto, CA), 13 B27 supplement (Gibco Media), 10 nmol/L gastrin (Sigma-Aldrich), 1 mmol/L N-acetylcysteine (Sigma-Aldrich), 13HEPES buffer (Gibco Media), and 13N2 supplement (GibcoMedia).

    Article Title:
    Article Snippet: Related to Figure 1C, D. Experiment number (stage 1) 1 2 3 4 5 St ag e 1 a BE1 + 0.1% BSA 1 day 100 ng/mL Activin A (Peprotech) 2 μM CHIR99021 (Axon MedChem) 3 μM CHIR 20 ng/μL Wnt3A (PeproTech) St ag e 1 b BE1 + 0.1% BSA 2 days 3 days 100 ng/mL Activin A 5 ng/mL FGF2 (Novoprotein) Supplemental Table S2.

    Article Title: BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways
    Article Snippet: After Matrigel polymerization for 10 min at 37 °C, taste bud organoid culture medium based on DMEM/F12 medium (Gibco) supplemented with Wnt3a (100 ng/mL, PeproTech, Cranbury, NJ, USA), R-spondin-1 (200 ng/mL, R&D systems, Minneapolis, MN, USA), Noggin (100 ng/mL, PeproTech), Y27632 (10 μM), N-acetylcysteine (1 mM, Sigma), epidermal growth factor (50 ng/mL, PeproTech), N2 (1%, Gibco), and B27 (2%, Gibco) was added to the plate and replaced every 3 days.

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses.
    Article Snippet: EBs were treated with 5 ng ml−1 BMP4 for 1 d and then 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 6 ng ml−1 Activin A (PeproTech), 6 ng ml−1 WNT3A (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 25 ng ml−1 follistatin (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Bicinchoninic Acid Protein Assay:

    Article Title: Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
    Article Snippet: Briefly, frozen enteroids were thawed, washed, fragmented and suspended in ice-cold Matrigel (CorningTM) supplemented with the recombinant human growth factors Noggin (100 ng/mL; Peprotech), R-spondin (500 ng/mL; Peprotech), EGF (50 ng/mL; CorningTM), Wnt3a (100 ng/mL; Peprotech), Y-27632 (10 μM; Peprotech), SB202190 (10 μM; Tocris BioscienceTM), LY2157299 (500 nM; A ChemBlock) and CHIR99021 (2.5 μM; Tocris BioscienceTM).

    Article Title: TGFβ2-Driven Ferritin Degradation and Subsequent Ferroptosis Underlie Salivary Gland Dysfunction in Postmenopausal Conditions.
    Article Snippet: Wnt3a (100 ng mL−1; Peprotech) was added to growth media at early passages (P0–P2) to enhance stability, while SB was omitted for TGFβ2 treatment experiments.

    Article Title: Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration
    Article Snippet: The culture medium was changed every 3 days using EEO medium composed of DMEM/F12 (Gibco), penicillin/streptomycin (100 U/L; Gibco), HEPES (10 mM; Gibco), GlutaMAX (1×; Gibco) , B27 supplement (1×; Gibco), N2 supplement (1×; Gibco), ITS Liquid Media Supplement (1×; Sigma‒Aldrich), Nicotinamide (1 mM; Sigma‒Aldrich), N-acetylcysteine-1 (1.25 mM; Sigma‒Aldrich), EGF (50 ng/mL; Peprotech), FGF-10 (100 ng/mL; Peprotech), Wnt3a (200 ng/mL; Peprotech), Noggin (100 ng/mL; Peprotech), Rspondin-1 (200 ng/mL; Peprotech), Y-27632 (9 μM; AbMole BioScience), A83-01 (500 nM; AbMole BioScience), SB202190 (10 μM; AbMole BioScience).

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses
    Article Snippet: EBs were treated with 5 ng ml −1 BMP4 for 1 d and then 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 6 ng ml −1 Activin A (PeproTech), 6 ng ml −1 WNT3A (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 25 ng ml −1 follistatin (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Article Title: NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.
    Article Snippet: This medium was enriched with various growth factors and supplements: 50 ng/mL epidermal growth factor (Sigma-Aldrich), 100 ng/mL FGF10 (Peprotech), 100 ng/mL Wnt3a (Peprotech, Rocky Hill, NJ), 100 ng/mL Noggin (R&D Systems), 1 mg/mL R-spondin 1 (Antibody and Protein Resource at Vanderbilt, Palo Alto, CA), 13 B27 supplement (Gibco Media), 10 nmol/L gastrin (Sigma-Aldrich), 1 mmol/L N-acetylcysteine (Sigma-Aldrich), 13HEPES buffer (Gibco Media), and 13N2 supplement (GibcoMedia).

    Article Title:
    Article Snippet: Related to Figure 1C, D. Experiment number (stage 1) 1 2 3 4 5 St ag e 1 a BE1 + 0.1% BSA 1 day 100 ng/mL Activin A (Peprotech) 2 μM CHIR99021 (Axon MedChem) 3 μM CHIR 20 ng/μL Wnt3A (PeproTech) St ag e 1 b BE1 + 0.1% BSA 2 days 3 days 100 ng/mL Activin A 5 ng/mL FGF2 (Novoprotein) Supplemental Table S2.

    Article Title: BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways
    Article Snippet: After Matrigel polymerization for 10 min at 37 °C, taste bud organoid culture medium based on DMEM/F12 medium (Gibco) supplemented with Wnt3a (100 ng/mL, PeproTech, Cranbury, NJ, USA), R-spondin-1 (200 ng/mL, R&D systems, Minneapolis, MN, USA), Noggin (100 ng/mL, PeproTech), Y27632 (10 μM), N-acetylcysteine (1 mM, Sigma), epidermal growth factor (50 ng/mL, PeproTech), N2 (1%, Gibco), and B27 (2%, Gibco) was added to the plate and replaced every 3 days.

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses.
    Article Snippet: EBs were treated with 5 ng ml−1 BMP4 for 1 d and then 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 6 ng ml−1 Activin A (PeproTech), 6 ng ml−1 WNT3A (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 25 ng ml−1 follistatin (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Cell Recovery:

    Article Title: Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
    Article Snippet: Briefly, frozen enteroids were thawed, washed, fragmented and suspended in ice-cold Matrigel (CorningTM) supplemented with the recombinant human growth factors Noggin (100 ng/mL; Peprotech), R-spondin (500 ng/mL; Peprotech), EGF (50 ng/mL; CorningTM), Wnt3a (100 ng/mL; Peprotech), Y-27632 (10 μM; Peprotech), SB202190 (10 μM; Tocris BioscienceTM), LY2157299 (500 nM; A ChemBlock) and CHIR99021 (2.5 μM; Tocris BioscienceTM).

    Article Title: TGFβ2-Driven Ferritin Degradation and Subsequent Ferroptosis Underlie Salivary Gland Dysfunction in Postmenopausal Conditions.
    Article Snippet: Wnt3a (100 ng mL−1; Peprotech) was added to growth media at early passages (P0–P2) to enhance stability, while SB was omitted for TGFβ2 treatment experiments.

    Article Title: Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration
    Article Snippet: The culture medium was changed every 3 days using EEO medium composed of DMEM/F12 (Gibco), penicillin/streptomycin (100 U/L; Gibco), HEPES (10 mM; Gibco), GlutaMAX (1×; Gibco) , B27 supplement (1×; Gibco), N2 supplement (1×; Gibco), ITS Liquid Media Supplement (1×; Sigma‒Aldrich), Nicotinamide (1 mM; Sigma‒Aldrich), N-acetylcysteine-1 (1.25 mM; Sigma‒Aldrich), EGF (50 ng/mL; Peprotech), FGF-10 (100 ng/mL; Peprotech), Wnt3a (200 ng/mL; Peprotech), Noggin (100 ng/mL; Peprotech), Rspondin-1 (200 ng/mL; Peprotech), Y-27632 (9 μM; AbMole BioScience), A83-01 (500 nM; AbMole BioScience), SB202190 (10 μM; AbMole BioScience).

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses
    Article Snippet: EBs were treated with 5 ng ml −1 BMP4 for 1 d and then 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 6 ng ml −1 Activin A (PeproTech), 6 ng ml −1 WNT3A (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 25 ng ml −1 follistatin (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Article Title: NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.
    Article Snippet: This medium was enriched with various growth factors and supplements: 50 ng/mL epidermal growth factor (Sigma-Aldrich), 100 ng/mL FGF10 (Peprotech), 100 ng/mL Wnt3a (Peprotech, Rocky Hill, NJ), 100 ng/mL Noggin (R&D Systems), 1 mg/mL R-spondin 1 (Antibody and Protein Resource at Vanderbilt, Palo Alto, CA), 13 B27 supplement (Gibco Media), 10 nmol/L gastrin (Sigma-Aldrich), 1 mmol/L N-acetylcysteine (Sigma-Aldrich), 13HEPES buffer (Gibco Media), and 13N2 supplement (GibcoMedia).

    Article Title:
    Article Snippet: Related to Figure 1C, D. Experiment number (stage 1) 1 2 3 4 5 St ag e 1 a BE1 + 0.1% BSA 1 day 100 ng/mL Activin A (Peprotech) 2 μM CHIR99021 (Axon MedChem) 3 μM CHIR 20 ng/μL Wnt3A (PeproTech) St ag e 1 b BE1 + 0.1% BSA 2 days 3 days 100 ng/mL Activin A 5 ng/mL FGF2 (Novoprotein) Supplemental Table S2.

    Article Title: BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways
    Article Snippet: After Matrigel polymerization for 10 min at 37 °C, taste bud organoid culture medium based on DMEM/F12 medium (Gibco) supplemented with Wnt3a (100 ng/mL, PeproTech, Cranbury, NJ, USA), R-spondin-1 (200 ng/mL, R&D systems, Minneapolis, MN, USA), Noggin (100 ng/mL, PeproTech), Y27632 (10 μM), N-acetylcysteine (1 mM, Sigma), epidermal growth factor (50 ng/mL, PeproTech), N2 (1%, Gibco), and B27 (2%, Gibco) was added to the plate and replaced every 3 days.

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses.
    Article Snippet: EBs were treated with 5 ng ml−1 BMP4 for 1 d and then 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 6 ng ml−1 Activin A (PeproTech), 6 ng ml−1 WNT3A (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 25 ng ml−1 follistatin (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    CCK-8 Assay:

    Article Title: Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
    Article Snippet: Briefly, frozen enteroids were thawed, washed, fragmented and suspended in ice-cold Matrigel (CorningTM) supplemented with the recombinant human growth factors Noggin (100 ng/mL; Peprotech), R-spondin (500 ng/mL; Peprotech), EGF (50 ng/mL; CorningTM), Wnt3a (100 ng/mL; Peprotech), Y-27632 (10 μM; Peprotech), SB202190 (10 μM; Tocris BioscienceTM), LY2157299 (500 nM; A ChemBlock) and CHIR99021 (2.5 μM; Tocris BioscienceTM).

    Article Title: TGFβ2-Driven Ferritin Degradation and Subsequent Ferroptosis Underlie Salivary Gland Dysfunction in Postmenopausal Conditions.
    Article Snippet: Wnt3a (100 ng mL−1; Peprotech) was added to growth media at early passages (P0–P2) to enhance stability, while SB was omitted for TGFβ2 treatment experiments.

    Article Title: Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration
    Article Snippet: The culture medium was changed every 3 days using EEO medium composed of DMEM/F12 (Gibco), penicillin/streptomycin (100 U/L; Gibco), HEPES (10 mM; Gibco), GlutaMAX (1×; Gibco) , B27 supplement (1×; Gibco), N2 supplement (1×; Gibco), ITS Liquid Media Supplement (1×; Sigma‒Aldrich), Nicotinamide (1 mM; Sigma‒Aldrich), N-acetylcysteine-1 (1.25 mM; Sigma‒Aldrich), EGF (50 ng/mL; Peprotech), FGF-10 (100 ng/mL; Peprotech), Wnt3a (200 ng/mL; Peprotech), Noggin (100 ng/mL; Peprotech), Rspondin-1 (200 ng/mL; Peprotech), Y-27632 (9 μM; AbMole BioScience), A83-01 (500 nM; AbMole BioScience), SB202190 (10 μM; AbMole BioScience).

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses
    Article Snippet: EBs were treated with 5 ng ml −1 BMP4 for 1 d and then 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 6 ng ml −1 Activin A (PeproTech), 6 ng ml −1 WNT3A (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 25 ng ml −1 follistatin (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Article Title: NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.
    Article Snippet: This medium was enriched with various growth factors and supplements: 50 ng/mL epidermal growth factor (Sigma-Aldrich), 100 ng/mL FGF10 (Peprotech), 100 ng/mL Wnt3a (Peprotech, Rocky Hill, NJ), 100 ng/mL Noggin (R&D Systems), 1 mg/mL R-spondin 1 (Antibody and Protein Resource at Vanderbilt, Palo Alto, CA), 13 B27 supplement (Gibco Media), 10 nmol/L gastrin (Sigma-Aldrich), 1 mmol/L N-acetylcysteine (Sigma-Aldrich), 13HEPES buffer (Gibco Media), and 13N2 supplement (GibcoMedia).

    Article Title:
    Article Snippet: Related to Figure 1C, D. Experiment number (stage 1) 1 2 3 4 5 St ag e 1 a BE1 + 0.1% BSA 1 day 100 ng/mL Activin A (Peprotech) 2 μM CHIR99021 (Axon MedChem) 3 μM CHIR 20 ng/μL Wnt3A (PeproTech) St ag e 1 b BE1 + 0.1% BSA 2 days 3 days 100 ng/mL Activin A 5 ng/mL FGF2 (Novoprotein) Supplemental Table S2.

    Article Title: BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways
    Article Snippet: After Matrigel polymerization for 10 min at 37 °C, taste bud organoid culture medium based on DMEM/F12 medium (Gibco) supplemented with Wnt3a (100 ng/mL, PeproTech, Cranbury, NJ, USA), R-spondin-1 (200 ng/mL, R&D systems, Minneapolis, MN, USA), Noggin (100 ng/mL, PeproTech), Y27632 (10 μM), N-acetylcysteine (1 mM, Sigma), epidermal growth factor (50 ng/mL, PeproTech), N2 (1%, Gibco), and B27 (2%, Gibco) was added to the plate and replaced every 3 days.

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses.
    Article Snippet: EBs were treated with 5 ng ml−1 BMP4 for 1 d and then 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 6 ng ml−1 Activin A (PeproTech), 6 ng ml−1 WNT3A (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 25 ng ml−1 follistatin (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Cell Viability Assay:

    Article Title: Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
    Article Snippet: Briefly, frozen enteroids were thawed, washed, fragmented and suspended in ice-cold Matrigel (CorningTM) supplemented with the recombinant human growth factors Noggin (100 ng/mL; Peprotech), R-spondin (500 ng/mL; Peprotech), EGF (50 ng/mL; CorningTM), Wnt3a (100 ng/mL; Peprotech), Y-27632 (10 μM; Peprotech), SB202190 (10 μM; Tocris BioscienceTM), LY2157299 (500 nM; A ChemBlock) and CHIR99021 (2.5 μM; Tocris BioscienceTM).

    Article Title: TGFβ2-Driven Ferritin Degradation and Subsequent Ferroptosis Underlie Salivary Gland Dysfunction in Postmenopausal Conditions.
    Article Snippet: Wnt3a (100 ng mL−1; Peprotech) was added to growth media at early passages (P0–P2) to enhance stability, while SB was omitted for TGFβ2 treatment experiments.

    Article Title: Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration
    Article Snippet: The culture medium was changed every 3 days using EEO medium composed of DMEM/F12 (Gibco), penicillin/streptomycin (100 U/L; Gibco), HEPES (10 mM; Gibco), GlutaMAX (1×; Gibco) , B27 supplement (1×; Gibco), N2 supplement (1×; Gibco), ITS Liquid Media Supplement (1×; Sigma‒Aldrich), Nicotinamide (1 mM; Sigma‒Aldrich), N-acetylcysteine-1 (1.25 mM; Sigma‒Aldrich), EGF (50 ng/mL; Peprotech), FGF-10 (100 ng/mL; Peprotech), Wnt3a (200 ng/mL; Peprotech), Noggin (100 ng/mL; Peprotech), Rspondin-1 (200 ng/mL; Peprotech), Y-27632 (9 μM; AbMole BioScience), A83-01 (500 nM; AbMole BioScience), SB202190 (10 μM; AbMole BioScience).

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses
    Article Snippet: EBs were treated with 5 ng ml −1 BMP4 for 1 d and then 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 6 ng ml −1 Activin A (PeproTech), 6 ng ml −1 WNT3A (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 25 ng ml −1 follistatin (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Article Title: NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.
    Article Snippet: This medium was enriched with various growth factors and supplements: 50 ng/mL epidermal growth factor (Sigma-Aldrich), 100 ng/mL FGF10 (Peprotech), 100 ng/mL Wnt3a (Peprotech, Rocky Hill, NJ), 100 ng/mL Noggin (R&D Systems), 1 mg/mL R-spondin 1 (Antibody and Protein Resource at Vanderbilt, Palo Alto, CA), 13 B27 supplement (Gibco Media), 10 nmol/L gastrin (Sigma-Aldrich), 1 mmol/L N-acetylcysteine (Sigma-Aldrich), 13HEPES buffer (Gibco Media), and 13N2 supplement (GibcoMedia).

    Article Title:
    Article Snippet: Related to Figure 1C, D. Experiment number (stage 1) 1 2 3 4 5 St ag e 1 a BE1 + 0.1% BSA 1 day 100 ng/mL Activin A (Peprotech) 2 μM CHIR99021 (Axon MedChem) 3 μM CHIR 20 ng/μL Wnt3A (PeproTech) St ag e 1 b BE1 + 0.1% BSA 2 days 3 days 100 ng/mL Activin A 5 ng/mL FGF2 (Novoprotein) Supplemental Table S2.

    Article Title: BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways
    Article Snippet: After Matrigel polymerization for 10 min at 37 °C, taste bud organoid culture medium based on DMEM/F12 medium (Gibco) supplemented with Wnt3a (100 ng/mL, PeproTech, Cranbury, NJ, USA), R-spondin-1 (200 ng/mL, R&D systems, Minneapolis, MN, USA), Noggin (100 ng/mL, PeproTech), Y27632 (10 μM), N-acetylcysteine (1 mM, Sigma), epidermal growth factor (50 ng/mL, PeproTech), N2 (1%, Gibco), and B27 (2%, Gibco) was added to the plate and replaced every 3 days.

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses.
    Article Snippet: EBs were treated with 5 ng ml−1 BMP4 for 1 d and then 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 6 ng ml−1 Activin A (PeproTech), 6 ng ml−1 WNT3A (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 25 ng ml−1 follistatin (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Injection:

    Article Title: Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
    Article Snippet: Briefly, frozen enteroids were thawed, washed, fragmented and suspended in ice-cold Matrigel (CorningTM) supplemented with the recombinant human growth factors Noggin (100 ng/mL; Peprotech), R-spondin (500 ng/mL; Peprotech), EGF (50 ng/mL; CorningTM), Wnt3a (100 ng/mL; Peprotech), Y-27632 (10 μM; Peprotech), SB202190 (10 μM; Tocris BioscienceTM), LY2157299 (500 nM; A ChemBlock) and CHIR99021 (2.5 μM; Tocris BioscienceTM).

    Article Title: TGFβ2-Driven Ferritin Degradation and Subsequent Ferroptosis Underlie Salivary Gland Dysfunction in Postmenopausal Conditions.
    Article Snippet: Wnt3a (100 ng mL−1; Peprotech) was added to growth media at early passages (P0–P2) to enhance stability, while SB was omitted for TGFβ2 treatment experiments.

    Article Title: Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration
    Article Snippet: The culture medium was changed every 3 days using EEO medium composed of DMEM/F12 (Gibco), penicillin/streptomycin (100 U/L; Gibco), HEPES (10 mM; Gibco), GlutaMAX (1×; Gibco) , B27 supplement (1×; Gibco), N2 supplement (1×; Gibco), ITS Liquid Media Supplement (1×; Sigma‒Aldrich), Nicotinamide (1 mM; Sigma‒Aldrich), N-acetylcysteine-1 (1.25 mM; Sigma‒Aldrich), EGF (50 ng/mL; Peprotech), FGF-10 (100 ng/mL; Peprotech), Wnt3a (200 ng/mL; Peprotech), Noggin (100 ng/mL; Peprotech), Rspondin-1 (200 ng/mL; Peprotech), Y-27632 (9 μM; AbMole BioScience), A83-01 (500 nM; AbMole BioScience), SB202190 (10 μM; AbMole BioScience).

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses
    Article Snippet: EBs were treated with 5 ng ml −1 BMP4 for 1 d and then 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 6 ng ml −1 Activin A (PeproTech), 6 ng ml −1 WNT3A (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 25 ng ml −1 follistatin (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Article Title: NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.
    Article Snippet: This medium was enriched with various growth factors and supplements: 50 ng/mL epidermal growth factor (Sigma-Aldrich), 100 ng/mL FGF10 (Peprotech), 100 ng/mL Wnt3a (Peprotech, Rocky Hill, NJ), 100 ng/mL Noggin (R&D Systems), 1 mg/mL R-spondin 1 (Antibody and Protein Resource at Vanderbilt, Palo Alto, CA), 13 B27 supplement (Gibco Media), 10 nmol/L gastrin (Sigma-Aldrich), 1 mmol/L N-acetylcysteine (Sigma-Aldrich), 13HEPES buffer (Gibco Media), and 13N2 supplement (GibcoMedia).

    Article Title:
    Article Snippet: Related to Figure 1C, D. Experiment number (stage 1) 1 2 3 4 5 St ag e 1 a BE1 + 0.1% BSA 1 day 100 ng/mL Activin A (Peprotech) 2 μM CHIR99021 (Axon MedChem) 3 μM CHIR 20 ng/μL Wnt3A (PeproTech) St ag e 1 b BE1 + 0.1% BSA 2 days 3 days 100 ng/mL Activin A 5 ng/mL FGF2 (Novoprotein) Supplemental Table S2.

    Article Title: BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways
    Article Snippet: After Matrigel polymerization for 10 min at 37 °C, taste bud organoid culture medium based on DMEM/F12 medium (Gibco) supplemented with Wnt3a (100 ng/mL, PeproTech, Cranbury, NJ, USA), R-spondin-1 (200 ng/mL, R&D systems, Minneapolis, MN, USA), Noggin (100 ng/mL, PeproTech), Y27632 (10 μM), N-acetylcysteine (1 mM, Sigma), epidermal growth factor (50 ng/mL, PeproTech), N2 (1%, Gibco), and B27 (2%, Gibco) was added to the plate and replaced every 3 days.

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses.
    Article Snippet: EBs were treated with 5 ng ml−1 BMP4 for 1 d and then 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 6 ng ml−1 Activin A (PeproTech), 6 ng ml−1 WNT3A (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 25 ng ml−1 follistatin (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Software:

    Article Title: Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
    Article Snippet: Briefly, frozen enteroids were thawed, washed, fragmented and suspended in ice-cold Matrigel (CorningTM) supplemented with the recombinant human growth factors Noggin (100 ng/mL; Peprotech), R-spondin (500 ng/mL; Peprotech), EGF (50 ng/mL; CorningTM), Wnt3a (100 ng/mL; Peprotech), Y-27632 (10 μM; Peprotech), SB202190 (10 μM; Tocris BioscienceTM), LY2157299 (500 nM; A ChemBlock) and CHIR99021 (2.5 μM; Tocris BioscienceTM).

    Article Title: TGFβ2-Driven Ferritin Degradation and Subsequent Ferroptosis Underlie Salivary Gland Dysfunction in Postmenopausal Conditions.
    Article Snippet: Wnt3a (100 ng mL−1; Peprotech) was added to growth media at early passages (P0–P2) to enhance stability, while SB was omitted for TGFβ2 treatment experiments.

    Article Title: Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration
    Article Snippet: The culture medium was changed every 3 days using EEO medium composed of DMEM/F12 (Gibco), penicillin/streptomycin (100 U/L; Gibco), HEPES (10 mM; Gibco), GlutaMAX (1×; Gibco) , B27 supplement (1×; Gibco), N2 supplement (1×; Gibco), ITS Liquid Media Supplement (1×; Sigma‒Aldrich), Nicotinamide (1 mM; Sigma‒Aldrich), N-acetylcysteine-1 (1.25 mM; Sigma‒Aldrich), EGF (50 ng/mL; Peprotech), FGF-10 (100 ng/mL; Peprotech), Wnt3a (200 ng/mL; Peprotech), Noggin (100 ng/mL; Peprotech), Rspondin-1 (200 ng/mL; Peprotech), Y-27632 (9 μM; AbMole BioScience), A83-01 (500 nM; AbMole BioScience), SB202190 (10 μM; AbMole BioScience).

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses
    Article Snippet: EBs were treated with 5 ng ml −1 BMP4 for 1 d and then 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 6 ng ml −1 Activin A (PeproTech), 6 ng ml −1 WNT3A (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml −1 BMP4 (Thermo Fisher Scientific), 25 ng ml −1 follistatin (PeproTech) and 5 ng ml −1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.

    Article Title: NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.
    Article Snippet: This medium was enriched with various growth factors and supplements: 50 ng/mL epidermal growth factor (Sigma-Aldrich), 100 ng/mL FGF10 (Peprotech), 100 ng/mL Wnt3a (Peprotech, Rocky Hill, NJ), 100 ng/mL Noggin (R&D Systems), 1 mg/mL R-spondin 1 (Antibody and Protein Resource at Vanderbilt, Palo Alto, CA), 13 B27 supplement (Gibco Media), 10 nmol/L gastrin (Sigma-Aldrich), 1 mmol/L N-acetylcysteine (Sigma-Aldrich), 13HEPES buffer (Gibco Media), and 13N2 supplement (GibcoMedia).

    Article Title:
    Article Snippet: Related to Figure 1C, D. Experiment number (stage 1) 1 2 3 4 5 St ag e 1 a BE1 + 0.1% BSA 1 day 100 ng/mL Activin A (Peprotech) 2 μM CHIR99021 (Axon MedChem) 3 μM CHIR 20 ng/μL Wnt3A (PeproTech) St ag e 1 b BE1 + 0.1% BSA 2 days 3 days 100 ng/mL Activin A 5 ng/mL FGF2 (Novoprotein) Supplemental Table S2.

    Article Title: BuZhong YiQi Formula Alleviates Taste Disorders in Rats with Type 2 Diabetes Mellitus by Increasing the Number of Taste Buds and the Expression of Signaling Molecules in Taste Transduction Pathways
    Article Snippet: After Matrigel polymerization for 10 min at 37 °C, taste bud organoid culture medium based on DMEM/F12 medium (Gibco) supplemented with Wnt3a (100 ng/mL, PeproTech, Cranbury, NJ, USA), R-spondin-1 (200 ng/mL, R&D systems, Minneapolis, MN, USA), Noggin (100 ng/mL, PeproTech), Y27632 (10 μM), N-acetylcysteine (1 mM, Sigma), epidermal growth factor (50 ng/mL, PeproTech), N2 (1%, Gibco), and B27 (2%, Gibco) was added to the plate and replaced every 3 days.

    Article Title: Molecular and genetic insights into human ovarian aging from single-nuclei multi-omics analyses.
    Article Snippet: EBs were treated with 5 ng ml−1 BMP4 for 1 d and then 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 6 ng ml−1 Activin A (PeproTech), 6 ng ml−1 WNT3A (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 3 d. Afterwards, EBs were transferred to a 0.1% gelatin-coated plate and treated with 10 ng ml−1 BMP4 (Thermo Fisher Scientific), 25 ng ml−1 follistatin (PeproTech) and 5 ng ml−1 FGF2 (Thermo Fisher Scientific) for 10 d before collection.



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